Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Recent trends in protease-catalyzed peptide synthesis.

C Lombard1, J Saulnier, J M Wallach

  • 1Laboratoire de Biochimie Analytique et Synthèse Bioorganique, UFR Chimie-Biochimie, Université Claude Bernard Lyon 1, 43 bd du 11 Novembre 1918, 69622 Villeurbanne Cedex France.

Protein and Peptide Letters
|March 9, 2006
PubMed
Summary

Enzymatic peptide synthesis, using proteases, offers stereospecific advantages but faces limitations. Recent advancements focus on enzyme engineering and novel reaction conditions to overcome these challenges for broader industrial application.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Severely reduced antioxidant and impaired mitochondrial biomarkers could be linked to post-tuberculosis lung disease in a cohort from South Africa.

African journal of thoracic and critical care medicine·2026
Same author

Prevalence of alcohol use, associated risk factors, and the impact on the HIV care cascade among adolescent girls and young women in South Africa: Findings from the HERStory Study.

South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·2026
Same author

Mitochondrial respiratory analysis of cryopreserved PBMCs isolated from human blood.

Cryobiology·2025
Same author

25 years of Journal of Veterinary Cardiology: when it all started.

Journal of veterinary cardiology : the official journal of the European Society of Veterinary Cardiology·2024
Same author

Depression and post-traumatic stress symptoms two years post-rape and the role of early counselling: Rape Impact Cohort Evaluation (RICE) study.

European journal of psychotraumatology·2023
Same author

PI*ZQ0<sub>Attikon</sub> genotype discovery in severe alpha-1 antitrypsin deficiency.

Pulmonology·2023

Area of Science:

  • Biocatalysis
  • Enzymology
  • Organic Synthesis

Background:

  • Enzymatic peptide synthesis can be thermodynamically or kinetically controlled, with the latter limited to serine and cysteine proteases.
  • Current industrial applications are limited, with only aspartame production and insulin conversion widely established.
  • Existing methods offer stereospecificity and avoid side chain protection but have drawbacks hindering broader use.

Purpose of the Study:

  • To review recent advancements in enzymatic peptide synthesis.
  • To highlight new strategies for overcoming limitations in enzymatic peptide synthesis.
  • To provide examples of innovative approaches in recent literature.

Main Methods:

  • Exploring proteases with enhanced specificity.

Related Experiment Videos

  • Employing protease engineering to favor synthesis over hydrolysis.
  • Developing mimetic or inverse substrates to prevent product degradation.
  • Investigating changes in reactant physical states, including solid-state reactions and enzyme immobilization (e.g., cross-linked enzyme crystals).
  • Modifying experimental conditions, such as heterogeneous catalysis, low-water organic solvents, ionic liquids, and subzero temperatures.

    • Main Results:

    • Significant improvements in protease specificity and engineering for synthesis.
    • Development of strategies to limit further hydrolysis of synthesized peptides.
    • Successful application of altered physical states and reaction conditions, including heterogeneous catalysis and low-water environments.
    • Demonstration of feasibility through examples in recent scientific literature.

    Conclusions:

    • Recent innovations are expanding the scope and efficiency of enzymatic peptide synthesis.
    • New approaches in enzyme engineering, substrate design, and reaction conditions are crucial for industrial viability.
    • The field is moving towards more robust and versatile enzymatic peptide synthesis methodologies.