Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Controlling RNA self-assembly to form filaments.

Lorena Nasalean1, Stéphanie Baudrey, Neocles B Leontis

  • 1Department of Chemistry, Bowling Green State University, OH 43402, USA.

Nucleic Acids Research
|March 9, 2006
PubMed
Summary

Researchers designed RNA molecules (tectoRNAs) that self-assemble into complex, three-dimensional structures. These RNA assemblies mimic protein filaments, offering new possibilities for biomaterials and nanotechnology.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Function behind choreography: cytoskeletal, nuclear, and mechanical dynamics drive growth transition in root hair development.

The Plant cell·2026
Same author

Microfluidics to Follow Spatiotemporal Dynamics at the Nucleo-Cytoplasmic Interface During Plant Root Growth.

Methods in molecular biology (Clifton, N.J.)·2024
Same author

In planta imaging of pyridine nucleotides using second-generation fluorescent protein biosensors.

The Plant journal : for cell and molecular biology·2024
Same author

Droplet Surface Immunoassay by Relocation (D-SIRe) for High-Throughput Analysis of Cytosolic Proteins at the Single-Cell Level.

Analytical chemistry·2023
Same author

High-Throughput Development and Optimization of RNA-Based Fluorogenic Biosensors of Small Molecules Using Droplet-Based Microfluidics.

Methods in molecular biology (Clifton, N.J.)·2022
Same author

Droplet-Based Microfluidic Chip Design, Fabrication, and Use for Ultrahigh-Throughput DNA Analysis and Quantification.

Advances in experimental medicine and biology·2022

Area of Science:

  • Nanoscience and Nanotechnology
  • Biomolecular Engineering
  • Synthetic Biology

Background:

  • Controlling matter's organization at the nanoscale is crucial for nanoscience.
  • RNA tectonics utilizes modular RNA units (tectoRNAs) for programmed self-assembly into desired architectures.

Purpose of the Study:

  • To design three-dimensional tectoRNAs with programmable interaction interfaces.
  • To achieve conformational, topological, and orientational control over RNA self-assembly.
  • To engineer micrometer-scale RNA filaments resembling protein actin filaments.

Main Methods:

  • Incorporation of modular 4-way junction (4WJ) motifs and hairpin loops into tectoRNAs.
  • Design of specific and directional RNA-RNA interaction interfaces.
  • Utilizing supra-molecular equilibrium polymerization for filament formation.

Related Experiment Videos

Main Results:

  • Successfully designed tectoRNAs capable of controlled 3D self-assembly.
  • Achieved precise control over tectoRNA assembly through motif positioning within 4WJ structures.
  • Generated micrometer-scale RNA filaments with orientational compensation, mimicking actin filaments.

Conclusions:

  • Demonstrated the potential of RNA as a versatile scaffold for creating novel biomaterials.
  • Highlighted the programmability of RNA self-assembly for engineering complex nanostructures.
  • Opened avenues for biomimetic design of materials inspired by cytoskeletal proteins.