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Crystal structure of the DUF16 domain of MPN010 from Mycoplasma pneumoniae

Dong Hae Shin1, Jeong-Sun Kim, Hisao Yokota

  • 1College of Pharmacy, Ewha Womans Unversity, Seoul 120-750, Korea.

Protein Science : a Publication of the Protein Society
|March 9, 2006
PubMed

Insights

Researchers determined the crystal structure of the DUF16 domain from Mycoplasma pneumoniae. This analysis revealed unique structural features of its homotrimeric coiled-coils, suggesting a probable molecular function for this unknown protein domain.

Area of Science:

  • Structural Biology
  • Mycoplasma pneumoniae research
  • Protein domain analysis

Background:

  • The DUF16 domain, encoded by MPN010 in Mycoplasma pneumoniae, is a domain of unknown function.
  • Understanding the structure of such domains is crucial for elucidating their biological roles.

Purpose of the Study:

  • To determine the high-resolution crystal structure of the DUF16 domain.
  • To characterize the structural features of the DUF16 domain and compare them to known coiled-coil structures.
  • To propose a probable molecular function for the DUF16 family based on structural and phylogenetic analyses.

Main Methods:

  • X-ray crystallography to determine the crystal structure at 1.8 Å resolution.
  • Sequence analysis and secondary structure prediction.
  • Phylogenetic analysis of the DUF16 family.

Main Results:

  • The crystal structure of the DUF16 domain revealed two distinct homotrimeric coiled-coils.
  • The longer coiled-coil exhibits unique structural regions, including a phenylalanine-rich segment and a conserved glutamine-rich region.
  • Phylogenetic analysis identified five subclasses within the DUF16 family based on N-terminal sequences.

Conclusions:

  • The DUF16 domain possesses a unique homotrimeric coiled-coil structure with distinct regions.
  • Structural insights provide a basis for inferring the probable molecular function of the DUF16 family.
  • Further research can explore the functional implications of these structural findings in Mycoplasma pneumoniae.

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