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Updated: Aug 10, 2026

Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary
Published on: January 16, 2017
Flow-through partial-filling affinity capillary electrophoresis using a crossreactive antibody for enantiomeric
Nenad M Grubor1, Daniel W Armstrong, Ryszard Jankowiak
1Ames Laboratory - USDOE, Department of Chemistry, Iowa State University, Ames, Iowa, IA, USA.
Abstract:
It is demonstrated that the separation of diastereoisomers and enantiomers can be accomplished by the flow-through partial-filling affinity CE using a crossreactive mAb. This approach revealed differences in the binding strength of the (+/-)-cis- and (+/-)-trans-benzo[a]pyrene tetrols with the anti-polycyclic aromatic hydrocarbon mAb and demonstrated that (+)-enantiomers are more strongly immunocomplexed than their (-)-counterparts. It is proposed that crossreactive monoclonal antibodies (i.e. mAb raised against achiral molecule and possessing limited selectivity) could be effectively utilized for specific stereoisomeric differentiation and chiral separations.
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