Related Experiment Video
Updated: Aug 10, 2026

Full-Field Optical Coherence Microscopy for Histology-Like Analysis of Stromal Features in Corneal Grafts
Published on: October 21, 2022
Confocal microscopy of corneal flap microfolds after LASIK
Manuel Ramírez1, Everardo Hernández-Quintela, Valeria Sánchez-Huerta
1Cornea and Refractive Surgery Services, Asociación Para Evitar la Ceguera en México, Hospital "Luis Sánchez Bulnes," Universidad Nacional Autónoma de México, México City, México. mramirezf@medicasur.org.mx
Purpose:
To describe the morphological characteristics of microfolds that appear at the corneal flap after LASIK, as seen under confocal microscopy.
Methods:
Twenty-one eyes that had undergone LASIK were examined, all within 3 weeks to 1 month after surgery. A central scan of the total corneal thickness was obtained by using confocal microscopy in vivo. Confocal images were captured and digitized. The longitudinal orientation (vertical, horizontal, and oblique) and morphological characteristics of the microfolds were described and recorded.
Results:
Six eyes had folds at the central corneal flap, visible as linear distortions in the confocal images: one fold had a vertical orientation, two were horizontal, and three were oblique. The folds were visible from the epithelial basal cell layer to the stromal portion of the flap and were deeper than Bowman's layer.
Conclusions:
Confocal microscopy allowed visualization of microfolds after LASIK. With the appropriate software, it is possible to analyze the morphological characteristics of these folds. Flap microfolds after LASIK are deeper than Bowman's layer.
More Related Videos
11:29Corneal Confocal Microscopy: A Novel Non-invasive Technique to Quantify Small Fibre Pathology in Peripheral Neuropathies
Published on: January 3, 2011
12:36A Human Corneal Organ Culture Model of Descemet's Stripping Only with Accelerated Healing Stimulated by Engineered Fibroblast Growth Factor 1
Published on: July 22, 2022