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Updated: Aug 10, 2026

Electrophoretic Separation of Proteins
Published on: June 12, 2008
Original paper application of cyclodextrins as modifiers in electrophoretic separation of metalloporphyrins
Krystyna Pyrzyńska1, Krzysztof Kilian
1Department of Chemistry, Warsaw University, Pasteura, Warsaw, Poland. kryspyrz@chem.uw.edu.pl
Abstract:
Several metallocomplexes of tetrakis-carboxyphenylporphyrin (TCPP) were separated on fused-silica capillary using CZE with UV-VIS detection. Metalloporphyrins of Co(II), Cu(II), Mn(II), Ni(II), and Zn(II) were formed directly in TCPP solution with addition of Cd(II) to increase the formation reaction rate. The composition of BGE, its concentration, and pH were optimized to ensure the stability of complexes and proper resolution. In particular, the problem of signals' shape was investigated and discussed. The presence of beta-CD in borate buffer significantly improved separation efficiency and signal shapes due to formation of inclusion complexes. Under the best separation conditions (50 mM borate running buffer at pH 9 with addition of 2 mM beta-CD, 30 kV applied voltage) a separation of metal complexes with TCPP was accomplished in 16 min.
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