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[Estimation of postmortem interval using myofibril fragmentation index]
Rong-qi Wu1, Zi-qin Zhao, Yi-wen Shen
1Department of Forensic Medicine, Shanghai Medical College, Fudan University, Shanghai 200032,China. wurongqi@gmail.com
Objective:
To study the relationship between postmortem interval and the myofibril fragmentation index of skeletal muscle.
Methods:
Rabbit skeletal muscle were left in the room temperature for different postmortem intervals, and the protein concentration of each sample was detected by using biuret method. Furthermore, the myofibril fragmentation index of each sample was measured under the protein concentration level of 0.5 mg/mL.
Results:
The myofibril fragmentation index increased obviously according to the postmortem interval prolongation.
Conclusion:
The myofibril fragmentation index may be used on the estimation of early postmortem interval.
Insights
The myofibril fragmentation index in skeletal muscle increases with postmortem interval. This index shows promise for estimating early postmortem intervals in forensic science.
Area of Science:
- Forensic Science
- Biochemistry
- Muscle Physiology
Context:
- Determining the postmortem interval is crucial in forensic investigations.
- Skeletal muscle undergoes degradation postmortem, affecting its structural integrity.
- Existing methods for postmortem interval estimation have limitations, especially in early stages.
Purpose:
- To investigate the correlation between the postmortem interval and the myofibril fragmentation index (MFI) in skeletal muscle.
- To assess the potential of MFI as a biomarker for early postmortem interval estimation.
Summary:
- Rabbit skeletal muscle samples were analyzed at various postmortem intervals.
- Protein concentration was determined using the biuret method.
- The myofibril fragmentation index was measured at a standardized protein concentration (0.5 mg/mL).
- Results demonstrated a clear increase in MFI with prolonged postmortem intervals.
Impact:
- The myofibril fragmentation index may serve as a reliable indicator for estimating early postmortem intervals.
- This finding could enhance the accuracy and efficiency of forensic time-of-death estimations.
- Provides a biochemical marker for postmortem degradation processes in muscle tissue.
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