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Detection of Copy Number Alterations Using Single Cell Sequencing
Published on: February 17, 2017
[Study on application of the whole genome amplification in LCN]
1Shanghai Institute of Criminal Science and Technology, Shanghai 200083,China.
Fa Yi Xue Za Zhi
|March 10, 2006
Abstract
Objective:
To establish a method for whole genome amplification (WGA) based on (multiple displacement amplification MDA), and achieve DNA analysis of the human genome from low copy number (LCN).
Methods:
DNA sample was used for WGA according to the REPLI-g kit protocol (QIAGEN, Germany). WGA product was used for DNA analysis according to the Applied Biosystems Profiler Plus kit protocol (ABI, USA).
Results:
WGA product of DNA sample (10 pg) can be used for STR genotyping.
Conclusion:
WGA technology could be helpful for LCN DNA analysis.
