Related Experiment Video

Updated: Aug 10, 2026

Subcutaneous Angiotensin II Infusion using Osmotic Pumps Induces Aortic Aneurysms in Mice
07:21

Subcutaneous Angiotensin II Infusion using Osmotic Pumps Induces Aortic Aneurysms in Mice

Published on: September 28, 2015

Adrenomedullin in mast cells of abdominal aortic aneurysm

Toshihiro Tsuruda1, Johji Kato, Kinta Hatakeyama

  • 1First Department of Internal Medicine, Miyazaki Medical College, University of Miyazaki, Japan. ttsuruda@med.miyazaki-u.ac.jp

Cardiovascular Research
|March 10, 2006
PubMed
Abstract

Insights

Adrenomedullin (AM) from mast cells may protect against abdominal aortic aneurysm (AAA) development. This study found AM reduced collagen synthesis in AAA models, suggesting an anti-fibrotic role in AAA pathogenesis.

Area of Science:

  • Cardiovascular Biology
  • Vascular Remodeling
  • Atherosclerosis Research

Background:

  • Adrenomedullin (AM) is produced by vascular walls and has antifibrotic effects.
  • Cardiovascular remodeling involves complex cellular and molecular changes.
  • Human abdominal aortic aneurysm (AAA) is a serious vascular condition.

Purpose of the Study:

  • To investigate the pathophysiological role of adrenomedullin (AM) in human abdominal aortic aneurysm (AAA) development.
  • To explore the source and function of AM in the context of AAA.

Main Methods:

  • Immunohistochemical analysis of AM expression in atherosclerotic aortas and AAA tissues.
  • Quantification of mast cells in AAA and control samples.
  • In vitro studies using cultured human mast cells (HMC-1) and AAA fibroblasts.
  • Assessment of collagen synthesis via [3H]proline incorporation and procollagen type I C-peptide secretion.
  • Evaluation of synthetic AM and anti-AM monoclonal antibody effects on collagen synthesis.

Main Results:

  • Vascular smooth muscle cells showed AM positivity in early atherosclerosis.
  • Mast cells in AAA tissues exhibited intense AM immunoreactivity and were significantly increased compared to controls.
  • Cultured mast cells expressed AM gene and released AM peptide.
  • Synthetic AM reduced collagen synthesis in co-cultured mast cells and fibroblasts.
  • Blocking endogenous AM activity increased collagen synthesis.

Conclusions:

  • Mast cell-derived AM plays an anti-fibrotic role in abdominal aortic aneurysm (AAA) development.
  • This finding provides novel insights into the biological functions of mast cell-derived AM in AAA pathogenesis.

More Related Videos

Ultrasound Imaging of the Thoracic and Abdominal Aorta in Mice to Determine Aneurysm Dimensions06:08

Ultrasound Imaging of the Thoracic and Abdominal Aorta in Mice to Determine Aneurysm Dimensions

Published on: March 8, 2019

Related Concept Videos

Aneurysm I: Introduction01:30

Aneurysm I: Introduction

An aortic aneurysm is a localized outpouching or dilation at a weak point in the artery wall. It may involve different parts of the aorta, such as the abdominal aorta, aortic arch, or thoracic aorta.Etiological factorsSeveral disorders are associated with aortic aneurysms.Congenital causes, such as primary connective tissue disorders like Marfan syndrome, impact the integrity and strength of connective tissues, notably affecting the aorta. Marfan syndrome is a genetic disorder that specifically...
Aneurysm II: Clinical Manifestations and Diagnostic Studies01:21

Aneurysm II: Clinical Manifestations and Diagnostic Studies

Thoracic, aortic arch and abdominal aneurysms are significant vascular conditions that can present with various clinical manifestations and lead to serious complications. Understanding these manifestations and the appropriate diagnostic studies is essential for effective management and treatment.Thoracic Aortic AneurysmsThoracic aortic aneurysms often remain asymptomatic until they reach a size that impinges on adjacent structures. They typically cause deep, diffuse chest pain that radiates to...
Anatomy of the Adrenal Glands01:17

Anatomy of the Adrenal Glands

The adrenal or supra-renal glands, situated above the kidneys and aligned with the twelfth rib, are paired pyramid-shaped structures crucial for the body's stress response. During stress, these glands secrete hormones vital for adaptive physiological reactions.
These glands possess a distinctive yellow tinge due to the stored cholesterol and fatty acids required for hormone synthesis. They are encased in a fibrous capsule and cushioned by fat.
The adrenal gland comprises two distinct regions...
Aneurysm III: Interprofessional Care01:26

Aneurysm III: Interprofessional Care

Aneurysm management involves either conservative medical therapy or surgical intervention, depending on the size and symptoms of the aneurysm. Conservative management is generally reserved for smaller, asymptomatic aneurysms, while larger or symptomatic aneurysms often necessitate surgical repair.Conservative Medical TherapyFor small, asymptomatic aneurysms, particularly abdominal aortic aneurysms (AAA) less than 5.5 centimeters in diameter, conservative medical therapy is recommended. This...