Related Experiment Videos
The cytochrome chain of mitochondria exhibits variable H+/e- stoichiometry
S Papa1, N Capitanio, G Capitanio
1Institute of Medical Biochemistry and Chemistry, University of Bari, Italy.
FEBS Letters
|August 19, 1991
Abstract:
A study is presented of the ----H+/e- stoichiometry for H+ pumping by the cytochrome chain in isolated rat liver mitochondria under level-flow and steady-state conditions. It is shown that the ----H+/e- stoichiometry for the cytochrome chain varies under the influence of the flow rate and transmembrane delta microH+. The rate-dependence is shown to be associated with cytochrome c oxidase, whose ----H+/e- ratio varies from 0 to 1, whilst the ----H+/e- ratio for the span covered by cytochrome c reductase is invariably 2.