[The expression of hepatocyte growth factor in human dental pulp and dental pulp cells]
1West China School of Stomatology, Sichuan University, Chengdu 610041, Sichuan Province, China.
Purpose:
To study the expression of hepatocyte growth factor in the dental pulp cell cultured in vitro and the human's healthy dental pulp.
Methods:
The 4th generation dental pulp cell cultured in vitro and the extracted tooth for orthodontic therapy were used as study targets. The gene expressions of HGF and C-MET and the expression of C-METmRNA induced by HGF were detected by RT-PCR technique in dental pulp cell cultured in vitro. The expression of HGF was checked by immunohistochemical method in both dental pulp tissue and dental pulp cell cultured in vitro. SPSS10.0 software package was used for SNK-q test.
Results:
HGFmRNA and C-METmRNA could be detected in dental pulp cell cultured in vitro by real-time reserve transcriptase-polymerase chain reaction. HGF up regulated the expression of C-METmRNA. HGF was positively expressed in the cytoplasms of the dental pulp cell cultured in vitro whereas weak expression was seen in the odontoblast layer and vascular endothelium of the dental pulp tissue.
Conclusion:
HGF expresses by dental pulp cell cultured in vitro and dental pulp tissue of ex vivo healthy teeth.
