Related Experiment Videos
Liquid Tissue: proteomic profiling of formalin-fixed tissues.
DaRue A Prieto1, Brian L Hood, Marlene M Darfler
1SAIC-Frederick, Inc., National Cancer Institute, Frederick, MD, USA.
Biotechniques
|March 15, 2006
Summary
Mass spectrometry (MS) enables biomarker discovery in formalin-fixed paraffin-embedded (FFPE) tissues. This method allows for the identification and quantitation of proteins for clinical applications.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Biotechnology
Background:
- Clinical applications require validated protein biomarkers for diagnosis, prognosis, and therapy.
- Large-scale analysis of individual tissues is crucial for biomarker validation.
- Existing formalin-fixed paraffin-embedded (FFPE) tissue collections represent a valuable resource.
Purpose of the Study:
- To present a method for identifying and quantifying candidate biomarker proteins.
- To enable the use of FFPE tissues for proteomic analysis.
- To facilitate the validation of protein biomarkers for clinical use.
Main Methods:
- Utilizing Liquid Tissue MS protein preparation.
- Leveraging mass spectrometry (MS) for peptide analysis.
- Analyzing peptides derived from FFPE tissues across various MS platforms.
Main Results:
- Demonstrated the feasibility of protein identification and quantitation in FFPE tissues.
- Enabled the procurement of peptides from FFPE samples for MS analysis.
- Showcased the adaptability of the method across different MS platforms.
Conclusions:
- Liquid Tissue MS protein preparation is a viable approach for proteomic analysis of FFPE tissues.
- This methodology facilitates the large-scale validation of protein biomarkers.
- The approach supports the clinical translation of diagnostic, prognostic, and therapeutic markers.