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Related Experiment Videos

Direct in vitro electrospinning with polymer melts.

Paul D Dalton1, Kristina Klinkhammer, Jochen Salber

  • 1Department of Textile and Macromolecular Chemistry and Deutsches Wollforschungsinstitut, RWTH-Aachen, Pauwelsstrasse 8, Aachen, Germany, D 52074. dalton@dwi.rwth-aachen.de

Biomacromolecules
|March 15, 2006
PubMed
Summary

Melt electrospinning directly onto fibroblasts successfully created layered tissue constructs. This technique avoids cytotoxic solvents and promotes cell adhesion and spindle-shaped morphology, advancing tissue engineering scaffolds.

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Polymer Science

Background:

  • Solution electrospinning for tissue engineering often uses cytotoxic solvents.
  • Melt electrospinning offers an alternative, avoiding solvents and enabling collection on cells.
  • Developing layered tissue constructs requires precise control over fiber deposition and cell integration.

Purpose of the Study:

  • To investigate melt electrospinning of a PEO-block-PCL/PCL blend directly onto in vitro cultured fibroblasts.
  • To identify key parameters governing melt electrospinning for direct cell collection.
  • To assess cell vitality and behavior post-electrospinning for tissue construct development.

Main Methods:

  • Melt electrospinning of a PEO-block-PCL with PCL polymer blend was performed.

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  • Significant electrospinning parameters (electric field, pump rate, viscosity) were optimized.
  • In vitro cultured fibroblasts served as the direct collection target for electrospun fibers.
  • Cell vitality and morphology were assessed post-electrospinning.

    • Main Results:

    • Optimal parameters yielded homogeneous, small fibers with a high electric field and adjusted pump rate.
    • Melt electrospinning parameters (lower pump rate, higher viscosity) differ significantly from solution electrospinning.
    • Cell vitality was maintained throughout the direct in vitro electrospinning process.
    • Fibroblasts adhered to and elongated along the electrospun fibers, changing from a spread to spindle shape.

    Conclusions:

    • Direct in vitro electrospinning of polymer melts onto cells is a viable method for tissue engineering.
    • This technique successfully produced layered cell-polymer constructs, demonstrating potential for scaffold fabrication.
    • The process supports cell adhesion and promotes desired morphological changes for tissue integration.