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Related Experiment Videos

Biosensors based on membrane transport proteins.

H Kiefer1, B Klee, E John

  • 1Max-Planck-Institut für Biologie, Tübingen, FRG.

Biosensors & Bioelectronics
|January 1, 1991
PubMed
Summary

We developed novel biosensors using membrane proteins for detection. A prototype lactose sensor utilizes lactose permease to detect lactose via pH changes, demonstrating a new sensing approach.

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[Not Available].

Strahlentherapie und Onkologie : Organ der Deutschen Rontgengesellschaft ... [et al]·2016

Area of Science:

  • Biotechnology
  • Biosensor technology
  • Membrane protein engineering

Background:

  • Biosensors often rely on enzymes or antibodies as recognition elements.
  • Membrane-bound proteins offer unique sensing capabilities due to their specific binding and transport functions.
  • Existing biosensor designs may have limitations in stability or specificity.

Purpose of the Study:

  • To introduce a novel class of biosensors utilizing membrane-bound proteins as the core sensing element.
  • To demonstrate the feasibility of incorporating transport proteins into planar lipid bilayers for biosensing.
  • To develop and test a prototype biosensor for lactose detection.

Main Methods:

  • Incorporation of membrane proteins into a planar lipid bilayer covering a transducer.

Related Experiment Videos

  • Utilizing a quartz slide coated with a lipid membrane containing purified lactose permease from Escherichia coli.
  • Detection of lactose via monitoring pH changes using a pH-sensitive fluorescence dye, triggered by lactose/H+ cotransport.

    • Main Results:

    • Successful incorporation of lactose permease into a lipid bilayer membrane.
    • Demonstration of lactose-induced H+ cotransport across the membrane.
    • Detection of lactose by measuring the resultant change in proton concentration via fluorescence.

    Conclusions:

    • Membrane-bound transport proteins can serve as effective sensing elements in biosensors.
    • Planar lipid bilayer systems provide a viable platform for immobilizing and utilizing membrane proteins.
    • This approach offers a novel strategy for developing specific and sensitive biosensors for various analytes.