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Updated: Jul 9, 2026

In Vitro Reconstitution of Self-Organizing Protein Patterns on Supported Lipid Bilayers
Published on: July 28, 2018
A landmark protein essential for establishing and perpetuating the polarity of a bacterial cell
Hubert Lam1, Whitman B Schofield, Christine Jacobs-Wagner
1Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA.
Abstract:
Polarity is often an intrinsic property of the cell, yet little is known about its origin or its maintenance over generations. Here we identify a landmark protein, TipN, which acts as a spatial and temporal cue for setting up the correct polarity in the bacterium Caulobacter crescentus. TipN marks the new pole throughout most of the cell cycle, and its relocation to the nascent poles at the end of division provides a preexisting reference point for orienting the polarity axis in the progeny. Deletion of tipN causes pleiotropic polarity defects, including frequently reversed asymmetry in progeny size and mislocalization of proteins and organelles. Ectopic localization of TipN along the lateral side of the cell creates new axes of polarity leading to cell branching and formation of competent cell poles. Localization defects of the actin-like protein MreB in the DeltatipN mutant suggest that TipN is upstream of MreB in regulating cell polarity.
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