Related Experiment Video

Updated: Aug 10, 2026

Measuring Granulocyte and Monocyte Phagocytosis and Oxidative Burst Activity in Human Blood
11:29

Measuring Granulocyte and Monocyte Phagocytosis and Oxidative Burst Activity in Human Blood

Published on: September 12, 2016

Effects of CNI-1493 on human granulocyte functions

Hana Abdalla1, Tony Forslund, Thomas Schön

  • 1Department of Molecular and Clinical Medicine, Division of Medical Microbiology, Faculty of Health Sciences, Linköping University, S-581 85 Linköping, Sweden. hanab@imk.liu.se

Immunobiology
|March 15, 2006
PubMed

Insights

CNI-1493, a macrophage deactivator, reduces nitric oxide (NO) production in macrophages and alters granulocyte function. These effects may explain its survival benefits in bacterial infections like Haemophilus influenzae type b (Hib).

Area of Science:

  • Immunology
  • Pharmacology

Background:

  • Nitric oxide (NO) and inflammatory mediators are key in bacterial infections like sepsis and meningitis.
  • CNI-1493, a macrophage deactivator, previously reduced mortality in infant rats infected with Haemophilus influenzae type b (Hib).

Purpose of the Study:

  • To investigate the in vitro effects of CNI-1493 on granulocytes and macrophages.

Main Methods:

  • Murine macrophages (RAW 264.7) were pre-incubated with CNI-1493 before activation with lipopolysaccharide (LPS)/interferon gamma (IFNgamma).
  • Granulocyte function was assessed after CNI-1493 treatment and stimulation with formylmethionyl-leucyl-phenylalanine (FMLP).
  • Nitrite/nitrate levels and inducible NO-synthase (iNOS) expression were measured in macrophages.
  • Reactive oxygen species (ROS) production, F-actin content, motility, and chemotaxis were measured in granulocytes.

Main Results:

  • CNI-1493 decreased NO production and iNOS expression in activated macrophages.
  • CNI-1493 increased ROS production in FMLP-stimulated granulocytes.
  • CNI-1493 decreased F-actin content, motility, and chemotaxis in granulocytes.

Conclusions:

  • CNI-1493's effects on macrophage NO production and granulocyte function may contribute to reduced inflammation and improved survival in Hib-infected animals.
  • These findings elucidate the mechanisms underlying CNI-1493's therapeutic potential in bacterial infections.

More Related Videos

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells10:21

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Published on: February 21, 2018

A Detailed Protocol for Characterizing the Murine C1498 Cell Line and its Associated Leukemia Mouse Model08:00

A Detailed Protocol for Characterizing the Murine C1498 Cell Line and its Associated Leukemia Mouse Model

Published on: October 14, 2016

Related Concept Videos

Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...