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Weakly and strongly associated nonfreezable water bound in bones
V V Turov1, V M Gun'ko, V I Zarko
1Institute of Surface Chemistry, 17 General Naumov Street, 03164 Kiev, Ukraine.
Colloids and Surfaces. B, Biointerfaces
|March 15, 2006
Summary
Investigating bone water using 1H NMR spectroscopy and TSDC revealed two distinct water types: typical and unusual. Unusual water, interacting with hydrophobic bone environments, increases with overall bone water content.
Area of Science:
- Biophysics
- Materials Science
- Quantum Chemistry
Background:
- Bone tissue contains water crucial for its mechanical properties.
- Understanding the state and behavior of bone water is essential for bone health research.
Purpose of the Study:
- To characterize the different types of water present in bone tissue.
- To investigate the relationship between water content and its interaction within the bone matrix.
Main Methods:
- 1H Nuclear Magnetic Resonance (NMR) spectroscopy at 210-300 K.
- Thermally Stimulated Depolarization Current (TSDC) method at 190-265 K.
- Quantum chemical calculations (GIAO, B3LYP/6-31G(d,p)) and solvent effect analysis (HF/SM5.45/6-31G(d)).
Main Results:
- Two distinct proton resonance signals were observed in 1H NMR spectra of bone water: typical water (δH=4-5 ppm) and unusual water (δH=1.2-1.7 ppm).
- Unusual water is attributed to molecules not forming hydrogen bonds via their hydrogen atoms, potentially interacting with hydrophobic bone environments.
- Increased overall bone water content correlates with a higher proportion of typical water, which exhibits greater hydrogen bonding and occupies larger bone spaces.
Conclusions:
- Bone water exists in at least two distinct states, with implications for bone's structural integrity.
- The characterization of unusual water provides insights into water-bone matrix interactions.
- Water's role in bone biomechanics is influenced by its binding state and distribution within bone's porous structure.
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