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Updated: Aug 10, 2026

Measurement of Heme Synthesis Levels in Mammalian Cells
Published on: July 9, 2015
Hyperhomocysteinemia and functional cobalamin deficiency due to granulocytosis-induced alterations in the
Lodewijk Thomas Vlasveld1, Gerard M J Bos, Anton A M Ermens
1Department of Internal Medicine, Bronovo Hospital, Bronovolaan 5, 2597, AX The Hague, The Netherlands. tomvlasveld@hotmail.com
Abstract:
Serum cobalamin and homocysteine levels were studied in patients with chronic myelogenous leukemia (CML) and in stem cell donors treated with granulocyte-colony stimulating factor (G-CSF). Cytoreductive treatment in patients with CML resulted in a decrease of cobalamin and homocysteine levels. In stem cell donors cobalamin and homocysteine levels increased after G-CSF administration. The increase of homocysteine level was accompanied by a decrease in the serum levels of the cobalamin-binding protein transcobalamin. We hypothesize that the increased homocysteine levels in patients with CML and donors treated with G-CSF may be the result of a functional methylcobalamin deficiency due to decreased transcobalamin levels.
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