Related Experiment Video
Updated: Aug 9, 2026
Evaluation of Keratinocyte Proliferation on Two- and Three-dimensional Type I Collagen Substrates
Published on: April 22, 2019
Characteristics of iris and retinal pigment epithelial cells cultured on collagen type I membranes
Gabriele Thumann1, Arno Hueber, Sven Dinslage
1Department of Ophthalmology and IZKF BIOMAT., RHWTH Aachen University, Germany. G.Thumann@uni-koeln.de
Purpose:
Transplantation of pigment epithelial cells is a promising treatment modality to repair retinal damage in age-related macular degeneration. For this purpose, it is necessary to establish cell culture techniques that allow acquisition of proper functional and morphological characteristics by the cells to be transplanted.
Methods:
Primary retinal pigment epithelial (RPE) and iris pigment epithelial (IPE) cells grown to confluence on collagen membranes were examined for morphology, adhesion, proliferation, apoptosis, as well as viability after ex vivo transplantation.
Results:
Pigment epithelial cells adhere, proliferate, form monolayers, acquire differentiated properties, and remain viable during transplantation to the subretinal space.
Conclusions:
Pigment epithelial cells cultured on collagen membranes acquire differentiated characteristics and are amenable to be transplanted as cell monolayers.
