Related Experiment Video
Updated: Aug 10, 2026

A Flame-Free Method for Sterilizing C. elegans Picks, Spatulas, and Scalpels
Published on: March 4, 2022
[Comparison between the efficacies of two different methods of preparing Esmarch bandages for sterilization]
Ahmet Doğan1, Mehmet Albayrak, I Erhan Mumcuoğlu
1Department of Orthopedics and Traumatology (1. Ortopedi ve Travmatoloji Kliniği), Istanbul Training and Research Hospital, 34365 Sişli, Istanbul, Turkey. orthopaedy@hotmail.com
Objectives:
Sterile Esmarch bandages have a widespread use in orthopedic surgery. We evaluated the efficacy of two different methods of preparing Esmarch bandages for sterilization.
Methods:
Two groups of Esmarch bandages were used, each group consisting of 25 bandages. The size of the bandages was 6.3 cm in width and 450 cm in length. Before sterilization, the bandages were tightly rolled in one group and loosely folded at a length of 10 cm in the other. An indicator was inserted at every five layers of all bandages. Sterilization was evaluated by subjecting both groups to ethylene oxide cycles and the autoclave method. A standard orthopedic surgical set was used as a control during each sterilization.
Results:
Following sterilization with ethylene oxide, all the indicators were found sterile in both groups. Similarly, complete sterilization was obtained in the loosely folded bandages with the autoclave method. However, only the indicators placed in the first 15 layers and the fiftieth layer were fully sterile in the tightly rolled bandages after autoclaving. The number of sterile indicators in the remaining layers ranged from nine (36%) to 23 (92%).
Conclusion:
Ethylene oxide is a reliable technique for sterilizing Esmarch bandages. The bandages must be loosely folded, instead of tightly rolling, when the autoclave technique is used.
Related Concept Videos
Methods of Sterilization II: Chemical Methods
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
Methods of Sterilization I: Physical Methods
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
Antimicrobial Effectiveness
Cleaning, Sterilization, and Disinfection
Cleaning
The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...

