Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structural basis for DNA recognition and processing by UvrB.

James J Truglio1, Erkan Karakas, Benjamin Rhau

  • 1Department of Pharmacological Sciences, Stony Brook University, Stony Brook, New York 11794-5115, USA.

Nature Structural & Molecular Biology
|March 15, 2006
PubMed
Summary

The study reveals how UvrB protein binds DNA during nucleotide excision repair (NER). It shows UvrB

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Editors' Note.

DNA repair·2026
Same author

Conformational landscape and ligand-dependent clustering of the human type 2 IP<sub>3</sub> receptor.

Nature communications·2026
Same author

ATR kinase inhibitors induce mitochondrial fission in CD8<sup>+</sup> T cells and impair immune memory <i>in vivo</i>.

bioRxiv : the preprint server for biology·2026
Same author

A dynamic search mechanism enables APE1 to identify AP-sites in DNA.

Nucleic acids research·2026
Same author

Structure Merging Approach Leads to New Dual Potent and Selective USP25/USP28 Inhibitors.

Journal of medicinal chemistry·2026
Same author

Molecular basis of nick ligation in the nucleosome by DNA Ligase IIIα.

bioRxiv : the preprint server for biology·2026

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Structural Biology

Background:

  • Nucleotide excision repair (NER) is crucial for removing DNA damage.
  • UvrB protein is central to prokaryotic NER, mediating DNA damage recognition and repair synthesis.

Purpose of the Study:

  • To elucidate the structural mechanism of UvrB binding to double-stranded DNA.
  • To provide insights into the formation of the preincision complex in NER.

Main Methods:

  • X-ray crystallography to determine the structure of the UvrB-double-stranded DNA complex.

Main Results:

  • The first structure of a UvrB-double-stranded DNA complex is presented.
  • A beta-hairpin motif in UvrB inserts between DNA strands, locking either strand.

Related Experiment Videos

  • A flipped-out nucleotide binds to a conserved pocket in UvrB.

    • Conclusions:

    • The structure explains how UvrB recognizes and binds DNA during the NER pathway.
    • This mechanism is key for initiating DNA repair by forming the preincision complex.