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Separation of human serum lipoproteins into three major classes by hydroxyapatite chromatography
U Matsumoto1, H Nakayama, Y Shibusawa
1Division of Analytical Chemistry, Tokyo College of Pharmacy, Japan.
Journal of Chromatography
|May 3, 1991
Abstract:
The separation of normolipidemic male serum lipoprotein fraction, prepared by ultracentrifugal flotation, was studied on hydroxyapatite columns. Potassium phosphate buffers in the pH range 5.6-7.4 were evaluated as eluents. The three main classes of the lipoproteins (high density, low density and very low density) can be separated on the Tiselius-type hydroxyapatite (Bio-Gel HTP DNA grade) column by elution with 75, 250 and 300 mM potassium phosphate buffer (pH 7.4), respectively.