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Published on: October 26, 2016
[Studies on the preparation and characterization of immobilized neutral protease by carboxymethyl chitosan
Zhitao Jin1, Guohua Chen, Xiaoyun Liu
1Department of Chemistry and Chemical Engineering of Ocean University of China, Qingdao 266003, China.
Abstract:
AS1. 398 neutral protease was immobilized onto carboxymethyl chitosan with glutaraldehyde as cross-linking agent. The effects of pH, time of cross-linking, amount of cross-linking agent and the ratio of enzyme to carrier on the activity of the immobilized enzyme were demonstrated, and the optimum immobilization condition of AS1. 398 neutral protease was established. Also studied was the characterization of immobilized enzyme,including pH, temperature, Km and the stability of storage.
