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Sequence-enabled reassembly of beta-lactamase (SEER-LAC): a sensitive method for the detection of double-stranded DNA

Aik T Ooi1, Cliff I Stains, Indraneel Ghosh

  • 1Department of Pharmacology and Toxicology, University of Arizona, Tucson, Arizona 85721, USA.

Biochemistry
|March 15, 2006
PubMed

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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