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Novel RNA-Binding Proteins Isolation by the RaPID Methodology
Published on: September 30, 2016
NABP1, a novel RORgamma-regulated gene encoding a single-stranded nucleic-acid-binding protein
Hong Soon Kang1, Ju Youn Beak, Yong-Sik Kim
1Cell Biology Section, Laboratory of Respiratory Biology, Division of Intramural Research, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA.
The Biochemical Journal
|March 15, 2006
Summary
Retinoid-related orphan receptor gamma2 (RORgamma2) regulates thymopoiesis. A novel nucleic-acid-binding protein 1 (NABP1) was discovered, highly expressed in wild-type thymus and suppressed in RORgamma2-deficient mice, suggesting RORgamma2 regulates NABP1.
Area of Science:
- Immunology
- Molecular Biology
- Genetics
Background:
- Retinoid-related orphan receptor gamma2 (RORgamma2) is crucial for thymopoiesis.
- Gene expression differences in thymocytes between wild-type and RORgamma2-deficient mice were investigated.
Purpose of the Study:
- To identify novel genes regulated by RORgamma2 during thymopoiesis.
- To characterize the function and properties of a newly identified protein, NABP1.
Main Methods:
- Microarray analysis to compare gene expression profiles.
- Confocal microscopy for protein localization.
- Size-exclusion chromatography to analyze protein complexes.
- Nucleic acid binding assays.
Main Results:
- A novel gene encoding nucleic-acid-binding protein 1 (NABP1) was identified.
- NABP1 expression is high in wild-type thymus and suppressed in RORgamma2-deficient mice, correlating with RORgamma2 expression patterns.
- NABP1 localizes to the nucleus and binds single-stranded nucleic acids.
Conclusions:
- NABP1 is a novel RORgamma2-regulated protein involved in thymopoiesis.
- NABP1 functions as a single-stranded nucleic acid binding protein within a larger nuclear complex.
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