Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Thermal stability of proteins.

John C Bischof1, Xiaoming He

  • 1Department of Mechanical Engineering, University of Minnesota, Minneapolis, MN 55455, USA. bischof@tc.umn.edu

Annals of the New York Academy of Sciences
|March 15, 2006
PubMed
Summary

Protein stability is crucial for biomaterial applications. Understanding how temperature, chemical additives, pH, and mechanical stress affect protein denaturation kinetics, particularly hydration

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Retraction Note: Heterozygous deletion of chromosome 17p renders prostate cancer vulnerable to inhibition of RNA polymerase II.

Nature communications·2026
Same author

Retraction Note: Targeting 17q23 amplicon to overcome the resistance to anti-HER2 therapy in HER2+ breast cancer.

Nature communications·2026
Same author

Retraction Note: Targeted production of reactive oxygen species in mitochondria to overcome cancer drug resistance.

Nature communications·2026
Same author

Retraction Note: A biomimetic hybrid nanoplatform for encapsulation and precisely controlled delivery of theranostic agents.

Nature communications·2026
Same author

Sex-Specific Modifiable Factors for Incident Late-Onset Dementia in the Geographic Context: A Longitudinal Comparative Analysis of Multicohort Studies across 19 Countries.

Neuroepidemiology·2026
Same author

Retraction Note: Carbon nano-onion-mediated dual targeting of P-selectin and P-glycoprotein to overcome cancer drug resistance.

Nature communications·2026

Area of Science:

  • Biomaterials Science
  • Biophysics
  • Biochemistry

Background:

  • Protein stability is vital for thermal therapies, burn injury treatment, and biopreservation.
  • Understanding protein denaturation is key to controlling outcomes in thermally mediated applications.
  • Current research focuses on heat-induced denaturation, but cold-induced denaturation remains less understood.

Purpose of the Study:

  • To review protein stability, focusing on denaturation kinetics under various thermal and chemical conditions.
  • To explore the impact of chemical additives, pH, and mechanical stress on protein denaturation.
  • To highlight the importance of protein hydration in stability and denaturation processes.

Main Methods:

  • Literature review of protein denaturation.
  • Analysis of factors influencing denaturation kinetics (temperature, pH, chemical additives, mechanical stress).
  • Examination of the role of protein hydration in stability.

Main Results:

  • pH affects both activation energy and frequency factor of denaturation kinetics.
  • Chemical and mechanical modifiers primarily impact the frequency factor (activation entropy), often linked to hydration.
  • Heat-induced denaturation is well-studied, but denaturation of structural proteins in tissues and during freezing/drying requires further research.

Conclusions:

  • Protein hydration is a critical factor influencing stability and denaturation across different conditions.
  • Further research into the kinetics of protein denaturation during freezing and drying is essential for applications like cryosurgery and preservation.
  • A deeper understanding of protein hydration at multiple levels is crucial for advancing biomaterial applications.

Related Experiment Videos