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Development of a real-time PCR for the detection of Chlamydia psittaci

Armelle Ménard, Maithé Clerc, Agathe Subtil1

  • 1Unité de Biologie des Interactions Cellulaires, Institut Pasteur, CNRS URA 2582, Paris, France.

Journal of Medical Microbiology
|March 15, 2006
PubMed
Abstract

No abstract available in PubMed .

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...