Rapid and simultaneous determination of nonsteroidal anti-inflammatory drugs in human plasma by LC-MS with
Koichi Suenami1, Lee Wah Lim, Toyohide Takeuchi
1Department of Chemistry, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan. c18871@pref.gifu.lg.jp
Abstract:
A rapid, selective and sensitive analytical method for the simultaneous determination of 16 nonsteroidal anti-inflammatory drugs (NSAIDs) in human plasma was carried out using Oasis HLB solid-phase extraction (SPE), followed by reversed-phase high-performance liquid chromatography and quadrupole mass spectrometry with electrospray ionization operated in the negative ion mode. The recoveries of NSAIDs from human plasma by SPE were greater than 76.7%. The use of a short column packed with small particles enabled rapid and simultaneous determination within 7 min. The detection limits for the NSAIDs were 0.01-0.9 mug/ml using the selected ion monitoring mode.
