Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Carbohydrate Utilization in Lactobacillus sake.

R Lauret, F Morel-Deville, F Berthier

    Applied and Environmental Microbiology
    |June 1, 1996
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Hand and wrist Osteo-articular bone defect: induced membrane technique indications.

    Hand surgery & rehabilitation·2023
    Same author

    [Impact of AMARA VIEW™ interface on upper airway during nocturnal non invasive ventilation].

    Revue des maladies respiratoires·2021
    Same author

    Comment on: Application of a new serratus anterior plane block in modified radical mastectomy under ultrasound guidance: A prospective, randomized controlled trial.

    Journal of clinical anesthesia·2021
    Same author

    Efficient transformation of Lactobacillus sake by electroporation.

    Microbiology (Reading, England)·2021
    Same author

    Impact of two-level filtering on emergency medical communication center triage during the COVID-19 pandemic: an uncontrolled before-after study.

    Scandinavian journal of trauma, resuscitation and emergency medicine·2020
    Same author

    Analysis of Au-Pd driving forces via the effective site energy model: LRO, antisites and enthalpy of permutation.

    Journal of physics. Condensed matter : an Institute of Physics journal·2020
    Same journal

    A set of constitutive promoters with graded strengths for gene expression in diverse cyanobacterial strains.

    Applied and environmental microbiology·2026
    Same journal

    Sources and traits of bacteria and fungi found in the near-surface atmosphere.

    Applied and environmental microbiology·2026
    Same journal

    Advancing One Health in Africa through continental early warning environmental surveillance.

    Applied and environmental microbiology·2026
    Same journal

    Specificity and longevity of a bacterial interspecies mutual cooperation benefiting organic micropollutant biodegradation.

    Applied and environmental microbiology·2026
    Same journal

    Coordinated regulation of trimethylamine catabolism in abundant marine bacteria.

    Applied and environmental microbiology·2026
    Same journal

    Synergistic and individual effects of RNase E, II, and R in the regulation of <i>Escherichia coli</i> growth and metabolism.

    Applied and environmental microbiology·2026
    See all related articles

    Lactobacillus sake utilizes various sugars via the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS). Specific PTS permeases and alternative pathways facilitate nutrient uptake and metabolism in this bacterium.

    Area of Science:

    • Microbiology
    • Bacterial Physiology
    • Carbohydrate Metabolism

    Background:

    • Understanding nutrient utilization is crucial for bacterial growth and function.
    • Lactobacillus sake is a significant lactic acid bacterium with diverse metabolic capabilities.

    Purpose of the Study:

    • To investigate the carbon source utilization of Lactobacillus sake.
    • To elucidate the mechanisms of carbohydrate transport and phosphorylation in L. sake.

    Main Methods:

    • Development of a chemically defined growth medium for L. sake.
    • Isolation and characterization of 2-deoxy-d-glucose resistant mutants.
    • Analysis of growth rates, carbohydrate uptake, and phosphorylation.

    Main Results:

    Related Experiment Videos

    • L. sake utilizes sucrose, fructose, mannose, N-acetylglucosamine, and glucose primarily through the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS).
    • The mannose-specific PTS permease (enzyme II(supMan)) mediates transport of mannose, glucose, and N-acetylglucosamine.
    • A distinct non-PTS system facilitates glucose transport, followed by ATP-dependent phosphorylation; ribose and gluconate use PTS-independent permeases.

    Conclusions:

    • Lactobacillus sake employs a complex system of PTS and non-PTS transporters for carbohydrate acquisition.
    • Enzyme II(supMan) plays a central role in the uptake of multiple sugars.
    • The study reveals specific pathways for glucose metabolism and other sugar transport mechanisms in L. sake.