Related Experiment Video
Updated: Feb 9, 2026
08:12
Measuring the Effect of Chemicals on the Growth and Reproduction of Caenorhabditis elegans
Published on: October 5, 2017
18.5K
Development of a Chemically Defined Medium for the Growth of Leuconostoc mesenteroides
Applied and Environmental Microbiology
|January 1, 1997
Abstract:
A chemically defined medium for the growth of Leuconostoc mesenteroides was developed. This medium contained lactose, Mn(sup2+), Mg(sup2+), 12 amino acids, eight vitamins, adenine, uracil, and Tween 80. We showed the beneficial effect of aerobic conditions on growth and that potassium phosphate (135 mM) is a suitable buffer. The growth rate in this medium was 0.85 (plusmn) 0.10 h(sup-1) for the six strains examined, and cell densities up to 3.5 x 10(sup9) CFU/ml were attained.
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