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A nitrite microsensor for profiling environmental biofilms.

D De Beer, A Schramm, C M Santegoeds

    Applied and Environmental Microbiology
    |March 1, 1997
    PubMed
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    A new nitrite microsensor with a hydrophilic coating offers improved stability and lower detection limits for environmental monitoring. This enhanced sensor enables in situ measurements in complex samples like biofilms and bioreactors.

    Area of Science:

    • Analytical Chemistry
    • Environmental Science
    • Electrochemistry

    Background:

    • Nitrite (NO2-) monitoring is crucial in environmental and industrial processes.
    • Existing microsensors face challenges with stability and detection limits in complex matrices.
    • Hydrophobic ion-carriers are effective for selective ion sensing.

    Purpose of the Study:

    • To develop and characterize a highly selective liquid membrane nitrite microsensor.
    • To improve sensor robustness and performance for in situ measurements.
    • To enable nitrite detection in challenging environments like biofilms and bioreactors.

    Main Methods:

    • Fabrication of a polyvinyl chloride (PVC)-gelled liquid membrane microsensor using aquocyanocobalt(III)-hepta(2-phenylethyl)-cobrynate.

    Related Experiment Videos

  • Application of a hydrophilic coating to the sensor tip.
  • Performance evaluation in freshwater and seawater, and in situ measurements in wastewater treatment plant biofilms.

    • Main Results:

    • The sensor exhibits log-linear response down to 1 µM NO2- in freshwater and 10 µM NO2- in seawater.
    • The hydrophilic coating and larger tip diameter (5-15 µm) enhance sensor sturdiness, stability, and lower detection limits compared to smaller sensors.
    • Successful in situ measurements in denitrifying, nitrifying, and nitrifying/denitrifying biofilms revealed high nitrite concentrations in narrow zones (<1 mm).

    Conclusions:

    • The novel coated nitrite microsensor is suitable for reliable in situ monitoring in complex environmental samples.
    • The sensor design overcomes limitations of previous microsensors, enabling application in bioreactors and biofilms.
    • This technology facilitates detailed spatial analysis of nitrogen cycling processes in microbial communities.