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Updated: Jun 25, 2026

Concurrent Quantification of Cellular and Extracellular Components of Biofilms
Published on: December 11, 2013
Combined light microscopy and attenuated total reflection fourier transform infrared spectroscopy for integration of
Abstract:
Reflected differential interference contrast microscopy and attenuated total reflection Fourier transform infrared spectroscopy were used to obtain complementary data on the structural and chemical properties of a biofilm. This information was obtained nondestructively, quasisimultaneously, and in real time, thereby permitting the verification of time-dependent relationships between the biofilm's population structure, distribution, and interfacial chemistry. The approach offers opportunities to examine these relationships on a variety of substrata in the presence of a bulk aqueous phase under controlled hydrodynamic conditions.
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