Related Experiment Video
Updated: Aug 10, 2026
DNA Vector-based RNA Interference to Study Gene Function in Cancer
Published on: June 4, 2012
[Construction of hPARP1-deficient cell strain by RNA interference]
Yan Sha1, Yu-yun Zeng, Zhi-xiong Zhuang
1Faculty of Preventive Medicine, School of Public Health, Sun Yet-san University, Guangzhou 510089, China.
Objective:
To construct hPARP1-deficient cell strain which can be used in studying the functions and action mechanisms of hPARP1 gene.
Methods:
16-HBEs were transfected with two kinds of eukaryotic expression plasmids of hPARP1 gene short hairpin RNA to construct hPARP1-deficient cells (named as "16-HBEP1 and 16-HBEP2") and a negative control plasmids (named as 16-HBEN). The protein expression levels of hPARP1 gene in 16-HBE, 16-HBEP1 16-HBEP2 and 16-HBEN were detected by the western blotting to estimate the effects of RNA interference.
Results:
The construction of hPARP1-deficient cell strain was successful and the protein expression level of hPARP1 gene in the 16-HBEP1 and 16-HBEP2 were decreased 84.3% and 63.7% respectively as compared with that in 16-HBE.
Conclusion:
The successes in constructing hPARP1-deficient strain offered an effective cell strain for studying the function of hPARP1 gene.
Related Concept Videos
02:15Experimental RNAi
01:23RNA Interference
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...