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Updated: Aug 10, 2026

Vinyl Chloride and High-Fat Diet as a Model of Environment and Obesity Interaction
Published on: January 12, 2020
[Study on the relationship between DNA damage and polymorphisms of metabolizing enzymes of vinyl chloride
Shou-min Zhu1, Wei Cheng, Feng-ren Du
1School of Public Health, Fudan University, Shanghai 200032, China.
Objective:
To explore the relationship between DNA damage induced by vinyl chloride monomer (VCM) and polymorphisms of metabolizing enzymes of VCM.
Methods:
Comet assay was employed to detect DNA damage, the workers were divided into two groups based on the status of DNA damage. Case-control design was used to investigate the relationship between the genetic polymorphisms of metabolizing enzymes and DNA damage. Genotypes of CYP2E1 c1/c2 and mEH4 His139Arg were identified by the PCR-RFLP, null genotypes of GST T1 and GST M1 were detected by PCR.
Results:
The genotypes of CYP2E1 c1c2 and c2c2 were significantly associated with DNA damages (P < 0.01). A prominent risk increasing of DNA damage was observed for those individuals having a high or low VCM exposure and possessing the CYP2E1 c1c2 and c2c2 genotypes (OR 4.92, 95% CI 1.35-13.85 and OR 2.57, 95% CI 1.01-6.59).
Conclusion:
Cumulative exposure dose and polymorphism of metabolizing enzymes may modulate the DNA damage of VCM-exposed workers. possessing the CYP2E1 c1c2 and c2c2 genotypes (OR 4.92, 95% CI 1.35-13.85 and OR 2.57, 95% CI 1.01-6.59).
Conclusion:
Cumulative exposure dose and polymorphism of metabolizing enzymes may modulate the DNA damage of VCM-exposed workers.
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