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[Preparation and characterization of immunogen against microcystin LR]
Xiao-lian Zhao1, Wei-rong Sun, Xiu-lan Sun
1Jiangsu Institute of Microbiology, Wuxi 214063, China.
Objective:
To prepare Microcystins-LR (MC-LR) conjugates with the carrier protein for developing a new immune assay for MC-LR.
Methods:
MC-LR was coupled to BSA or KLH by "activated ester" and "water-soluble carbodimide". Measure the combine ratio of MC-LR with KLH or BSA by the UV scanning or the Bradford's method.
Results:
The results are 9.1 and 9.4. New Zealand rabbits was immunized by MC-LR-KLH. The titer of the polyantibodies was over 25,600 measured by id ELISA.
Conclusion:
MC-LR-KLH was proved the suitable immunogen against MC-LR.
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