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Published on: January 7, 2014
[Expression of costimulator 4-1BBL and B7-1 on glioma cell lines]
Yong-Gao Mu1, Hui Peng, Jun-Ying Zhang
1State Key Laboratory of Oncology in South China, Guangzhou, Guangdong 510060, P. R. China.
Background & Objective:
Costimulatory molecules 4-1BBL and B7-1 have been proposed to be therapeutic targets to improve and sustain antitumor immune response. This study was to investigate the expression of 4-1BBL and B7-1 on several glioma cell lines.
Methods:
The expression of 4-1BBL and B7-1 on glioma cell lines T98G, MGR2, MGR1, SF767, SKMG1, SKMG4, and UW28 was detected by flow cytometry. The cytotoxic effects of vincristine (VCR) on the glioma cell lines were assessed with MTT assay. The correlation of 4-1BBL expression to drug resistance was analyzed.
Results:
The 7 glioma cell lines showed various levels of 4-1BBL expression, but none expressed B7-1. The positive rate of 4-1BBL was above 30% in T98G and MGR1 cells which were resistant to VCR; while it was below 10% in MGR2, SF767, SKMG1, SKMG4, and UW28 cells which were sensitive to VCR.
Conclusions:
All of the detected cell lines express 4-1BBL, but none expresses B7-1. The glioma cells with high 4-1BBL expression are resistant to VCR.