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Updated: Aug 10, 2026

Generation of Alginate Microspheres for Biomedical Applications
Published on: August 12, 2012
Applications of alginate in bioseparation of proteins
Sulakshana Jain1, Kalyani Mondal, Munishwar N Gupta
1Chemistry Department, Indian Institute of Technology, New Delhi, India.
Abstract:
Alginate is a polysaccharide that is a block polymer consisting of block units of guluronic acid and mannuronic acid. It shows inherent biological affinity for a variety of enzymes such as pectinase, lipase, phospholipase D, a and ss amylases and glucoamylase. Taking advantage of its precipitation with Ca2+ and the above-mentioned property, alginate has been used for purification of these enzymes by affinity precipitation, aqueous two phase separation, macroaffinity ligand facilitated three phase partitioning, immobilized metal affinity chromatography and expanded bed affinity chromatography. Thus, this versatile marine resource has tremendous potential in bioseparation of proteins.
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