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Related Experiment Videos

The mouse biliary glycoprotein gene (Bgp): partial nucleotide sequence, expression, and chromosomal assignment.

J Robbins1, P F Robbins, C A Kozak

  • 1Laboratory of Tumor Immunology and Biology, National Cancer Institute, Bethesda, Maryland 20892.

Genomics
|July 1, 1991
PubMed
Summary

Researchers identified a mouse biliary glycoprotein (BGP) gene fragment in mammary tumors. This gene fragment is homologous to human BGP and located on mouse chromosome 7, with related mRNA found in normal adult mouse tissues.

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Area of Science:

  • Molecular biology
  • Genetics
  • Cancer research

Background:

  • Carcinoembryonic antigen (CEA) related transcripts are present in induced mammary tumors.
  • A cDNA library from a dimethylbenzanthracene-induced tumor was used for clone isolation.

Purpose of the Study:

  • To identify and characterize CEA-related gene transcripts in mouse mammary tumors.
  • To determine the homology and chromosomal location of the identified mouse gene.

Main Methods:

  • cDNA library preparation and screening using a human CEA cDNA probe.
  • DNA sequencing and comparison with human CEA gene family members.
  • Southern and Northern blot analyses for gene localization and expression.

Main Results:

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  • A clone homologous to human biliary glycoprotein (BGP) was isolated, suggesting it encodes a portion of the mouse BGP gene.
  • Southern blot analysis indicated the presence of four restriction fragments on mouse chromosome 7.
  • Northern blot analysis revealed expression of related mRNA species in some normal adult mouse tissues.

Conclusions:

  • The study identified a mouse biliary glycoprotein (BGP) gene fragment within mammary tumors.
  • The mouse BGP gene is located on chromosome 7 and shows homology to its human counterpart.
  • The gene is expressed in normal adult mouse tissues, suggesting a potential role beyond tumorigenesis.