[Radioimmunological localization study using 131-iodine radioalbelled monoclone antibody ASC5 against human tongue...

Nude mice carrying human tongue squamous-cell carcinoma xenografts were studied in vivo.McAb ASC5 were purified from ascitic fluid obtained from BALB/c mice pristane-primed prior to being inocualted intraperitoneally with hybridoma cell,using with protein A sepharose CL-4B.Nude mice bearing xenografts were given i.v,injection of McAb labelled with 1311 and 1251.Mice were imaged daily with PHO gamma camera and sacrificed at interval after i.v.injection,weighted and counted.Gamma scanning indicated that there were a larger amount of activity detectable in tumor at 72h after i.v.injection 1311ASC5,then the tumor could be more clearly visualized at 96h and remained strongly position over the 7-day period,with the background activity decreasing.Tumor to non-tumor ratio was more than 2.9.It was secondary best of the antibody uptake at 72h,approximately 9.20+/-1.96%ID/g(mean+/-SD),which compared with isotypical antibody-paired normal mouse IgG,the difference was significant at P<0.05.Results demonstrated that ASC5 is useful in localization diagnosis and management of cancer.