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Related Experiment Videos

Characterization of Bves expression during mouse development using newly generated immunoreagents.

Travis K Smith1, David M Bader

  • 1The Stahlman Cardiovascular Research Laboratories, Program for Developmental Biology, and Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee 37232-6300, USA.

Developmental Dynamics : an Official Publication of the American Association of Anatomists
|March 16, 2006
PubMed
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Blood vessel/epicardial substance (Bves) is expressed in various muscle and epithelial cells during mouse development. This finding suggests a broad role for Bves in embryogenesis and warrants further functional investigation.

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Background:

  • Blood vessel/epicardial substance (Bves) is a transmembrane protein involved in cell-cell interactions.
  • Bves is highly expressed in the developing heart, but its expression in non-muscle cells is debated.
  • Understanding Bves expression is crucial for characterizing its developmental and adult functions.

Purpose of the Study:

  • To generate novel monoclonal antibodies against mouse Bves.
  • To elucidate the expression profile of Bves during mouse embryogenesis using these antibodies.

Main Methods:

  • Generation of monoclonal antibodies against mouse Bves.
  • Immunohistochemical analysis of Bves expression in developing mouse tissues.
  • In vitro studies of Bves expression in cultured cell lines.

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Main Results:

  • Bves is confirmed in developing heart myocytes.
  • Skeletal, smooth muscle cells, and coronary system cells express Bves.
  • Specific epithelial derivatives from all three germ layers show positive Bves staining.
  • In vitro cell line studies corroborate in vivo findings.

Conclusions:

  • Bves is broadly expressed in diverse epithelial and muscle cells during mouse embryogenesis.
  • These findings suggest a widespread function for Bves in development.
  • Newly developed Bves antibodies are valuable tools for future research.