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Related Experiment Videos

Starch industry wastewater-based stable Bacillus thuringiensis liquid formulations.

Satinder K Brar1, M Verma, R D Tyagi

  • 1INRS-ETE, Université du Québec, 490, Rue de la Couronne, Québec, Canada G1K 9A9.

Journal of Economic Entomology
|March 17, 2006
PubMed
Summary

Bacillus thuringiensis (Bt) liquid formulations from starch wastewater and soya medium showed stability for one year. Optimal suspending agents were identified, but formulations degraded at higher temperatures and pH.

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Area of Science:

  • Agricultural Science
  • Microbiology
  • Environmental Science

Background:

  • Bacillus thuringiensis (Bt) is a bacterium used as a biopesticide.
  • Developing stable liquid formulations is crucial for effective Bt application.
  • Starch industry wastewater (SIW) offers a potential low-cost fermentation medium.

Purpose of the Study:

  • To develop and assess the storage stability of liquid Bt formulations.
  • To evaluate the efficacy of different suspending agents.
  • To compare SIW-based Bt formulations with those from soya medium.

Main Methods:

  • Bt fermentation in SIW and soya medium.
  • Storage stability testing over one year under various pH and temperature conditions.
  • Analysis of physical, chemical, and biological parameters, including entomotoxicity.

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Main Results:

  • Sorbitol and sodium monophosphate (3:1 ratio) proved to be the most effective suspending agents.
  • SIW fermentation yielded higher Bt cell counts, spore counts, and entomotoxicity compared to soya medium.
  • Both formulations exhibited instability at pH > 6 and temperatures > 40°C.

Conclusions:

  • SIW is a viable and superior medium for Bt fermentation compared to soya medium.
  • Formulation stability is limited by pH and temperature, requiring controlled storage conditions.
  • Optimized suspending agents enhance the shelf-life and efficacy of Bt liquid formulations.