Related Experiment Video
Updated: Aug 10, 2026
Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries
Published on: January 22, 2019
Immuno-suppressive effect of blocking the CD28 signaling pathway in T-cells by an active component of Echinacea found
Guo-Chung Dong1, Ping-Hsien Chuang, Michael D Forrest
1Institute of BioAgricultural Sciences, Academia Sinica, Taipei, Taiwan 115, Republic of China.
Abstract:
AFTIR (after flowing through immobilized receptor) is a novel method for screening herbal extracts for pharmaceutical properties. Using AFTIR, we identified Cynarin in Echinacea purpurea by its selective binding to chip immobilized CD28, a receptor of T-cells, which is instrumental to immune functioning. The results of surface plasma resonance show that binding between immobilized CD28 and Cynarin is stronger than the binding between CD28 and CD80, a co-stimulated receptor of antigen presenting cells. Cynarin's function was verified by its ability to downregulate CD28-dependent interleukin-2 (IL-2) expression in a T-cell culture line. AFTIR offers promise as an efficient screening method for herbal medicines.
