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Toxin Induction and Protein Extraction from Fusarium spp. Cultures for Proteomic Studies
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[Extraction of multiple elements corresponding to Fasciola hepatica infestation]

Miguel Echenique-Elizondo1, José Antonio Amondarain-Arratíbel

  • 1Departamento de Cirugía, Facultad de Medicina, Universidad del País Vasco, San Sebastián, Spain.

Cirugia Espanola
|March 17, 2006
PubMed
Abstract

No abstract available in PubMed .

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Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is formed in...