Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Chromatin architecture.

Christopher L Woodcock1

  • 1Department of Biology and Program in Molecular and Cellular Biology, University of Massachusetts, Amherst, MA 01003, USA. chris@bio.umass.edu

Current Opinion in Structural Biology
|March 17, 2006
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hierarchical looping of zigzag nucleosome chains in metaphase chromosomes.

Proceedings of the National Academy of Sciences of the United States of America·2016
Same author

Sumoylated human histone H4 prevents chromatin compaction by inhibiting long-range internucleosomal interactions.

The Journal of biological chemistry·2014
Same author

Comparative structure of vertebrate sperm chromatin.

Journal of structural biology·2014
Same author

Human heterochromatin protein 1α promotes nucleosome associations that drive chromatin condensation.

The Journal of biological chemistry·2014
Same author

Nucleosome-free region dominates histone acetylation in targeting SWR1 to promoters for H2A.Z replacement.

Cell·2013
Same author

Chromatin organization - the 30 nm fiber.

Experimental cell research·2012
Same journal

Metabolic disruptions through a three-dimensional genomic lens.

Current opinion in structural biology·2026
Same journal

Collective variable design for biomolecular conformational dynamics.

Current opinion in structural biology·2026
Same journal

Polymer scaling in protein crowding: From dilute coils to semidilute meshes.

Current opinion in structural biology·2026
Same journal

Tuning the physicochemical properties of rationally designed protein-based biomolecular condensates.

Current opinion in structural biology·2026
Same journal

Editorial overview: Folding, binding and protein design.

Current opinion in structural biology·2026
Same journal

Macromolecular crowding reshapes the conformational landscapes of intrinsically disordered proteins: mechanisms, cellular contexts, and functional consequences.

Current opinion in structural biology·2026
See all related articles

Understanding chromatin structure requires moving beyond DNA sequence to 3D conformation. Advanced microscopy and spectroscopy reveal how histone variants and proteins influence this complex 3D structure, impacting the epigenome.

Area of Science:

  • Molecular Biology
  • Structural Biology
  • Epigenetics

Background:

  • Chromatin structure-function relationships are key to understanding gene regulation.
  • Current understanding relies heavily on one-dimensional DNA sequence information.
  • A comprehensive view necessitates incorporating three-dimensional conformational data.

Purpose of the Study:

  • To explore the three-dimensional conformation of chromatin.
  • To elucidate the roles of histone variants and proteins in chromatin structure.
  • To provide a basis for understanding the epigenome's conformation.

Main Methods:

  • In vitro assembly of nucleosomes and nucleosomal arrays.
  • X-ray diffraction
  • NMR spectroscopy

Related Experiment Videos

  • Electron microscopy
  • Atomic force microscopy

    • Main Results:

    • Detailed insights into the structural roles of histone variants.
    • Understanding the impact of specific histone mutations on chromatin.
    • Characterization of nucleosomal array compaction.
    • Apparent structural consequences of chromatin architectural protein binding.

    Conclusions:

    • Integrating 1D and 3D structural data is crucial for a complete understanding of chromatin.
    • Advanced biophysical techniques provide essential insights into chromatin organization.
    • These findings form a foundation for epigenome structure-function studies.