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Related Experiment Videos

Signal transduction in olfactory neurons.

N S Levy1, H A Bakalyar, R R Reed

  • 1Howard Hughes Medical Institute Laboratory of Genetics, Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205.

The Journal of Steroid Biochemistry and Molecular Biology
|October 1, 1991
PubMed
Summary

Researchers have cloned key molecules in the cyclic adenosine monophosphate (cAMP)-mediated olfactory signal transduction pathway, including olfactory receptors. This advance aids in understanding odorant receptor diversity and specificity.

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Area of Science:

  • Molecular biology
  • Neuroscience
  • Biochemistry

Background:

  • Olfactory signal transduction relies on a cAMP-mediated pathway.
  • Key components include G proteins, adenylyl cyclase, and cyclic nucleotide-gated cation channels.

Purpose of the Study:

  • To clone molecular components of olfactory signal transduction.
  • To identify and characterize olfactory receptors.

Main Methods:

  • Cloning of olfactory-specific G protein, adenylyl cyclase, and cation channel.
  • Functional expression of cloned proteins in vitro.
  • Generation of oligonucleotide probes for olfactory receptors.

Main Results:

  • Olfactory-specific G protein, adenylyl cyclase, and cation channel were isolated and functionally confirmed.

Related Experiment Videos

  • Putative odorant-receptor molecules were cloned.
  • Oligonucleotide probes revealed a heterogeneous population of olfactory receptors.

    • Conclusions:

    • The cloned components function within the cAMP-mediated olfactory pathway.
    • The cloned olfactory receptors are more diverse than previously known.
    • Probes will facilitate studies on receptor number and ligand specificity.