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Updated: Aug 10, 2026
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High Content Screening Analysis to Evaluate the Toxicological Effects of Harmful and Potentially Harmful Constituents (HPHC)
Published on: May 10, 2016
[Studies for analyzing the prohibited ingredients such as acetohexamide in cosmetics]
Hiroshi Tokunaga1, Tadashi Uchino
1tokunaga@nihs.go.jp
Summary
A new HPLC method accurately detects Acetohexamide (AH) in cosmetics. This validated analytical technique ensures compliance with cosmetic regulations by identifying prohibited ingredients.
Area of Science:
- Analytical Chemistry
- Cosmetic Science
Context:
- Acetohexamide (AH) is a prohibited ingredient in Japanese cosmetics under the Pharmaceutical Affairs Act.
- Accurate detection methods are crucial for ensuring cosmetic product safety and regulatory compliance.
Purpose:
- To develop and validate a High-Performance Liquid Chromatography (HPLC) method for the quantitative analysis of Acetohexamide (AH) in cosmetic formulations.
- To establish a reliable analytical procedure for detecting prohibited ingredients in lotions, milky lotions, and creams.
Summary:
- A robust HPLC method was established using an ODS column (CAPCELL PAK C18) with a mobile phase of acetonitrile and phosphate buffer (pH 5.3) at a 3:1 ratio, and detection at 247 nm.
- Sample preparation involved extraction and dilution with methanol for lotions/milky lotions, and dissolution with tetrahydrofuran followed by methanol for creams.
- The method demonstrated linearity between 0.5 and 6.0 microg/ml, with no interference from common cosmetic ingredients like parabens, confirming its specificity.
Impact:
- Provides a validated analytical tool for regulatory bodies and manufacturers to monitor Acetohexamide (AH) levels in cosmetics.
- Contributes to enhanced consumer safety by facilitating the detection of banned substances in cosmetic products.
- Supports the enforcement of cosmetic regulations in Japan and potentially other regions.
