Related Experiment Video
Updated: Aug 10, 2026

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
Published on: December 11, 2021
Rotationally selected product pair correlation: F+CD4 --> DF(nu')+CD3(nu2 = 0 and 2, N)
Jingang Zhou1, Weicheng Shiu, Jim J Lin
1The Institute of Atomic and Molecular Sciences, Academia Sinica, P.O. Box 23-166, Taipei 10617, Taiwan.
Abstract:
The product pair correlation of the title reaction was measured with rotational selection for both the vibrationally ground CD3(nu = 0) and umbrella-excited CD3(nu2 = 2) products. A striking linear relationship was found between the rotational energy of the selected CD3 product and the correlated kinetic energy release (or the average vibrational energy of the DF coproduct). Such a linearly correlated (or anticorrelated) dependence appears to be stronger for CD3(nu2 = 2,N) than for CD3(nu = 0,N). The mechanistic implication of the observation is that the rotational motion N of the CD3 product tends to lie antiparallel to the orbital angular momentum l' of the two departing products. The dependency on the K quantum number--the projection of N on the top axis--is, on the other hand, less significant yet noticeable.
Related Concept Videos
Interpreting ¹H NMR Signal Splitting: The (n + 1) Rule
2D NMR: Overview of Heteronuclear Correlation Techniques
2D NMR: Overview of Homonuclear Correlation Techniques
COSY90 is the standard two-dimensional (2D) COSY experiment that...
Spin–Spin Coupling: Three-Bond Coupling (Vicinal Coupling)
The extent of coupling depends on the C‑C bond length, the two H‑C‑C angles, any electron-withdrawing substituents, and the dihedral angle between the involved orbitals. The...
Coefficient of Correlation
If you suspect a linear relationship between x and y, then r can measure how strong the linear relationship is.
What the VALUE of r tells us:
The value of r is always between –1 and +1: –1 ≤ r ≤ 1.
The size of the correlation r indicates the strength of the linear...
Wilcoxon Signed-Ranks Test for Matched Pairs

