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Expression of FGF receptor gene in rat development
A Wanaka1, J Milbrandt, E M Johnson
1Department of Pharmacology, Washington University School of Medicine, St. Louis, Missouri 63110.
Development (Cambridge, England)
|February 11, 1991
Summary
Fibroblast Growth Factor Receptor (FGF-R) mRNA is expressed in various developing rat tissues, particularly in mesoderm and neuroectoderm-derived cells. FGF-R expression patterns are developmentally regulated, impacting neural and non-neural organ development.
Area of Science:
- Developmental Biology
- Molecular Biology
- Neuroscience
Background:
- Fibroblast Growth Factors (FGFs) are crucial signaling molecules regulating cell growth, differentiation, and development.
- FGF receptors (FGF-Rs) mediate FGF signaling, playing vital roles in embryonic development and tissue homeostasis.
Purpose of the Study:
- To investigate the spatiotemporal expression patterns of FGF-receptor (FGF-R) mRNA during rat embryonic and postnatal development.
- To identify specific tissues and cell types expressing FGF-R mRNA during key developmental stages.
Main Methods:
- In situ hybridization histochemistry was employed to detect FGF-R mRNA.
- Analysis was performed on embryonic tissues (E9, E12, E14, E17) and postnatal neural tissues (P1, P7, P14, adult).
Main Results:
- Significant FGF-R mRNA expression was detected in mesoderm- and neuroectoderm-derived tissues throughout development.
- In the developing nervous system, FGF-R mRNA was localized to the ependymal layer in embryos and specific neuronal populations (e.g., hippocampal neurons) postnatally.
- FGF-R mRNA expression was observed in sensory ganglia, retina, bone, kidney, and skin, with distinct developmental regulation in these tissues.
Conclusions:
- FGF-R gene expression is developmentally regulated and widespread in various embryonic and postnatal rat tissues.
- These findings support the role of FGF signaling in the development of diverse organ systems, including the nervous system, sensory organs, and skeletal structures.