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Published on: January 5, 2015
Highly sensitive spectrofluorimetric determination of trace amounts of lecithin using a norfloxacin-terbium probe
1Department of Chemistry, Shandong Normal University, Jinan, 250014, P R China.
Background:
Lecithin is found throughout biological systems. A new spectrofluorimetric method was developed for the determination of trace amounts of lecithin.
Methods:
Optimum conditions for the determination of lecithin were investigated.
Results:
Using norfloxacin (NF)-terbium ion (Tb3+) as a fluorescent probe, in a buffer solution at pH=6.80, lecithin reduced the fluorescence intensity of the NF-Tb3+ complex at lambda=545 nm; the reduced fluorescence intensity of the Tb3+ ion is proportional to the concentration of lecithin. The linear range and detection limit for the determination of lecithin were 1.08 x 10(-6)-3.02 x 10(-5) mol/l and 2.54 x 10(-7) mol/l.
Conclusion:
This method is simple, practical and relatively free of interference from coexisting substances, and can be successfully applied to assess lecithin in serum samples.
