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The temporal architecture of eukaryotic growth
David Lloyd1, Douglas B Murray
1Microbiology, Cardiff School of Biosciences, (BIOSI 1, Main Building), Cardiff University, P.O. Box 915, Cardiff CF10 3TL, Wales, UK. lloydd@cf.ac.uk
FEBS Letters
|March 21, 2006
Summary
Cellular redox state oscillations in Saccharomyces cerevisiae drive a synchronized transcriptome, orchestrating metabolism and cellular functions through a complex regulatory network.
Area of Science:
- Cellular dynamics and systems biology
- Microbial physiology
- Molecular biology
Background:
- Organismal development relies on precise temporal coordination.
- Cellular redox state fluctuations are critical for biological processes.
- Saccharomyces cerevisiae serves as a model organism for studying cellular dynamics.
Purpose of the Study:
- To investigate the oscillatory behavior of cellular redox state in Saccharomyces cerevisiae.
- To determine the relationship between redox oscillations and gene expression.
- To elucidate the network of transcriptional regulators involved in orchestrating cellular dynamics.
Main Methods:
- Continuous culture of Saccharomyces cerevisiae.
- Non-invasive monitoring of dissolved oxygen and NAD(P)H levels.
- Transcriptome-wide analysis of gene expression oscillations.
- Bioinformatic analysis of regulatory networks.
Main Results:
- Temperature-compensated oscillations in cellular redox state were observed, linked to respiratory cycles.
- The entire transcriptome exhibited low-amplitude oscillations.
- Transcripts related to metabolism, stress response, cellular structure, and DNA synthesis were identified as key oscillators.
- An intricate network of transcriptional regulators was found to orchestrate these oscillations.
Conclusions:
- Cellular auto-dynamism arises from a self-organized network of intracellular components.
- Mitochondrial, nuclear, transcriptional, and metabolic dynamics are coupled via the cellular redox state.
- This coordinated oscillatory behavior is essential for maintaining the temporal structure of growing organisms.
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